Ciliary nanoscale superstructures
Kloster Neustadt, 3DMM2O Meeting
March 5-6, 2024
Emerging marine model organisms
Image: Patrick Steinmetz
Nematostella vectensis
Platynereis dumerilii
Trichoplax adhaerens
Platynereis dumerilii
- breeding culture
- genome sequence
- microinjection, transgenesis
- neuron-specific promoters
- knock-out lines
- neuronal connectome
- neuronal activity imaging
Volume EM with Array Tomograpy
Array tomography workflow
Array tomography workflow
Array tomography workflow
Array tomography workflow
Synaptic connectome
Eukaryotic cilia
Emelie Brodrick
Ciliary superstructure 1 — metachronal waves
with Kirsty Wan, LSI Exeter
Distributed actuation and ionic regulation
Distributed actuation and ionic regulation
Ciliary superstructure 2 — pressure-sensing photoreceptors
Pressure response in Platynereis larvae
Ciliary photoreceptor cells sense Δ pressure
Microscopy pressure chamber 
Calcium imaging 
Photoreceptor responses to pressure are graded
Ciliary superstructure 3 — ctenophore balancer organ
Ciliary superstructure 3 — ctenophore balancer organ
- vEM reconstruction of the ctenophore apical organ
Ciliary superstructure 3 — ctenophore balancer organ
Ciliary superstructure 3 — ctenophore balancer organ
Ctenophore balancer — capacitative coupling?
Ctenophore balancer — capacitative coupling?
Acknowledgements
Heidelberg
- Alexandra Kerbl
- Sanja Jasek
- Emily Savage
- Simone Wolters
Exeter
- Kei Jokura
- Emelie Brodrick
- Luis A. Bezares-Calderón
- Luis Yanez
- Victoria Moris
- Daniel Thiel
- Adam Johnstone
