Nitric-oxide-feedback to ciliary photoreceptor cells orchestrates UV avoidance in a peptidergic circuit in Platynereis
Neuropeptide Signaling: Bridging Cell Biology, Neurophysiology, and Behavior
April 16 - 19, 2023
Emerging marine model organisms
Image: Patrick Steinmetz
Nematostella vectensis
Platynereis dumerilii
Trichoplax adhaerens
Placozoa – no synapses, many peptides
evolution of peptides and neurotransmitters
large-scale receptor deorphanisation
map chemical connectomes to scRNAseq
Large-scale GPCR-peptide screen in Nematostella
Mapping to single-cell data
Platynereis dumerilii
- breeding culture, full life-cycle
- embryos daily, year round
- genome sequence
- microinjection, transgenesis
- neuron-specific promoters and antibodies
- knock-out lines
- neuronal connectome
- whole-body neuronal activity imaging
- whole-animal pharmacology by bath application 😎
UV response in Platynereis larvae
UV-responding brain ciliary photoreceptors (cPRCs)
No UV avoidance in c-opsin1 knockouts
No cPRC response in c-opsin1 knockouts
Whole-body volume EM of an entire three-day-old larva
Synaptic connectome
Circuitry of ciliary photoreceptors
Circuitry of ciliary photoreceptors
Strong cPRC activation after UV exposure
Many peptides and modulators in the circuit
Multiplex immunogold for neuropeptides in the EM volume
Mapping of (pro)neuropeptides
Single-cell data for cPRC cells and their interneurons
::: footer Kei Jokura :::
Nitric-oxyde synthase in postsynaptic interneurons
HCR
Transgenic labelling
NO is produced in the neuropil after UV stimulation
NOS mutants have altered cPRC response
NOS mutants have altered INRGW and motoneuron response
NOS mutants show defective UV avoidance
Two unusual guanylyl cyclases in the cPRCs
NIT-GC1 RNA
NIT-GC1 protein
NIT-GC2 RNA
NIT-GC2 protein
Two unusual guanylyl cyclases in the cPRCs
NIT-GC morphants have altered circuit activity
Mathematical modelling of the circuit
Model fitting
wild type
NOS-11
NOS-23
NIT-GC2 mo.
Integration and memory of UV exposure
Acknowledgements
- Emelie Brodrick
- Cyrielle Kaltenrieder
- Réza Shahidi
- Milena Marinkovic
- Daniel Thiel
- Sanja Jasek
- Cameron Hird
- Rebecca Turner
- Luis A. Bezares-Calderón
- Kei Jokura
- Luis Yanez Guerra
- Alexandra Kerbl
Former lab members
- Albina Asadulina
- James Beard
- Markus Conzelmann
- Nadine Randel
- Philipp Bauknecht
- Martin Gühmann
- Cristina Pineiro-Lopez
- Nobuo Ueda
- Aurora Panzera
- Csaba Verasztó
- Elizabeth Williams
We have recently moved to Heidelberg
Postdoc openings - please get in touch!